• PMID: 1530367
• Gene Name: GANAB
• Condition: Azoospermia
• Association: Associated
• PMID: 1530367
• Gene Name: GANAB
• Condition: Azoopsermia
• Association: The seminal determination of alpha-glucosidase and, to a lesser extent, alpha-mannosidase and N-acetyl-beta-D-glucosaminidase helps rapidly, sensitivity, reliably, and noninvasively to differentiate secretory azoospermias (with higher enzymatic activity)
• Population size: 69
• Population details: 69 (8 normozoospermic controls, 7 secretory, and 54 excretory azoospermic subjects)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Azoopsermia

Enzyme and hormonal markers in the differential diagnosis of human azoospermia

García Díez LC, Esteban Ruiz PF, Villar E, Corrales Hernandez JJ, Burgo R, Delgado M, Miralles JM.

The serum concentrations of FSH, LH, prolactin, testosterone, and estradiol and the enzymatic activities of hyaluronidase, glucosidases (alpha-glucosidase, beta-glucosidase, alpha-mannosidase, N-acetyl-beta-D-glucosaminidase, beta-glucuronidase, and beta-galactosidase), lactate dehydrogenase and its isoenzymes (LDH1, LDH2, LDH3, LDH-X, LDH4), and total proteins were measured in the semen of 69 subjects (8 normozoospermic controls, 7 secretory, and 54 excretory azoospermic subjects). FSH levels rose with the deterioration in spermatogenesis and served to differentiate the secretory from the excretory azoospermias. The only source of hyaluronidase and LDH-X in the ejaculate is the spermatozoa. alpha-Glucosidase activity essentially originates in the epididymis. The seminal determination of alpha-glucosidase and, to a lesser extent, alpha-mannosidase and N-acetyl-beta-D-glucosaminidase helps rapidly, sensitivity, reliably, and noninvasively to differentiate secretory azoospermias (with higher enzymatic activity) from the excretory type (less enzymatic activity) and may be of use in identifying with a certain degree of reliability the site of obstruction in the male genital tract. FAU - García Díez, L C AU - García Díez LC AD - Servicio de Endocrinología, Hospital Clínico Universitario, Salamanca, Spain. FAU - Esteban Ruiz, P F AU - Esteban Ruiz PF FAU - Villar, E AU - Villar E FAU - Corrales Hernandez, J J AU - Corrales Hernandez JJ FAU - Burgo, R AU - Burgo R FAU - Delgado, M AU - Delgado M