• PMID: 14574809
• Gene Name: DAZ1
• Condition: Infertility
• Association: PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome
• Mutation: AZFc/DAZ microdeletions
• Population size: 117
• Population details: 117 (azoospermic and oligozoospermic)
• Infertility type: Male infertility

[Real-time fluorescent PCR for screening AZFc/DAZ microdeletions on the Y chromosome in male infertility patients]

Yu CY, Zhuang GL, Zhou CQ, Yan ZH, Li W, Gao H, Rui DR.

OBJECTIVE: To develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients. METHODS: A set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome. RESULTS: Eleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients. CONCLUSION: The real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR. FAU - Yu, Cong-Yi AU - Yu CY AD - Reproductive Medical Center, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, China. yucongyi01@sohu.com FAU - Zhuang, Guang-Lun AU - Zhuang GL FAU - Zhou, Can-Quan AU - Zhou CQ FAU - Yan, Zong-He AU - Yan ZH FAU - Li, Wei AU - Li W FAU - Gao, Hua AU - Gao H