• PMID: 11872225
• Gene Name: DAZL
• Condition: Sperm motility
• Association: The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in th
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Sperm motility

Presence of DAZL transcript and protein in mature human spermatozoa

Lin YM, Chen CW, Sun HS, Tsai SJ, Lin JS, Kuo PL.

OBJECTIVE: To identify the DAZL transcript and protein location in human spermatozoa. DESIGN: In vitro experiment. SETTING: University-based reproductive genetics laboratory. PATIENT(S): A fertile volunteer. INTERVENTION(S): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. MAIN OUTCOME MEASURE(S): Expression of DAZL in human spermatozoa. RESULT(S): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. CONCLUSION(S): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation. FAU - Lin, Yung Ming AU - Lin YM AD - Department of Urology, National Cheng Kung University, College of Medicine, Tainan, Taiwan. FAU - Chen, Chaio Wei AU - Chen CW FAU - Sun, H Sunny AU - Sun HS FAU - Tsai, Shaw Jeng AU - Tsai SJ FAU - Lin, Johnny Shinn Nan AU - Lin JS