• PMID: 11821083
• Gene Name: CYP11a
• Condition: PCOS
• Association: Associated

Overexpression of theca-cell messenger RNA in polycystic ovary syndrome does not correlate with polymorphisms in the cholesterol side-chain cleavage and 17alpha-hydroxylase/C(17-20) lyase promoters

Daneshmand S, Weitsman SR, Navab A, Jakimiuk AJ, Magoffin DA.

OBJECTIVE: To determine whether overexpression of CYP17 or CYP11A messenger (m)RNA in theca cells from polycystic ovaries is related to polymorphic regions in the gene promoters that may increase transcription. DESIGN: Case-control study. SETTING: Research institute. PATIENT(S): Fifty-one women with PCOS and 280 regularly cycling controls underwent genotyping. Thecal cells were obtained from 23 women with PCOS and 51 controls. MAIN OUTCOME MEASURE(S): Ovarian tissue was obtained from women with PCOS undergoing wedge resection for treatment of their infertility and from controls undergoing ovariectomy for indications unrelated to the study. Expression of mRNA in theca cells was measured by using competitive reverse transcriptase polymerase chain reaction. Genotype analysis for polymorphisms in the CYP11A and CYP17 promoters was performed by using polymerase chain reaction. RESULT(S): Although expression of CYP11A and CYP17 mRNA was higher in women with PCOS, no significant dose effects of CYP11A or CYP17 alleles were observed with respect to serum testosterone; follicular fluid androstenedione, estradiol, and androstenedione-to-estradiol ratio; or CYP11A or CYP17 mRNA expression. CONCLUSION(S): Overexpression of CYP17 and CYP11A mRNA in theca cells from polycystic ovaries is explained by polymorphic differences in the gene promoters. FAU - Daneshmand, Saíd AU - Daneshmand S AD - Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center Burns and Allen Research Institute, UCLA School of Medicine, 8700 Beverly Boulevard, Davis 2066, Los Angeles, CA 90048-0750, USA. FAU - Weitsman, Stacy R AU - Weitsman SR FAU - Navab, Alireza AU - Navab A FAU - Jakimiuk, Artur J AU - Jakimiuk AJ