• PMID: 11163833
• Gene Name: DAZ1
• Condition: Azoospermia
• Association: CDY1-minor expression was detected in all biopsies in which mature spermatids/spermatozoa were observed by histological analysis and/or in the minced tissue. CDY1-minor expression was also detected in two biopsies with arrest at the spermatocyte stage dur
• Population size: 66
• Population details: 66 men with azoospermia
• Sex: Male
• Infertility type: Male infertility
• Associated genes: DAZ, CDY1, RBM
• Other associated phenotypes: Azoospermia

Expression of CDY1 may identify complete spermatogenesis

Kleiman SE, Lagziel A, Yogev L, Botchan A, Paz G, Yavetz H.

OBJECTIVE: To investigate the expression of deleted in azoospermia (DAZ), RNA-binding motif (RBM), and chromodomain y1 (CDY1) genes in the testes of men with azoospermia with variable histopathologies. DESIGN: Prospective study. SETTING: Andrology laboratory of a university-affiliated maternity hospital. PATIENT(S): Sixty-six men with azoospermia. INTERVENTION(S): Testicular sperm extraction. MAIN OUTCOME MEASURE(S): The results of gene expression in testicular tissue tested by RT-PCR were correlated with those of histopathologically and microscopically examined minced testicular tissue. Y chromosome microdeletion testing and karyotyping were performed, as was direct sequencing of CDY1-PCR products. RESULT(S): CDY1-minor expression was detected in all biopsies in which mature spermatids/spermatozoa were observed by histological analysis and/or in the minced tissue. CDY1-minor expression was also detected in two biopsies with arrest at the spermatocyte stage during which no mature spermatids/spermatozoa were observed. A previously unreported CDY1-minor alternative splicing transcript was identified. DAZ and RBM gene expressions were detected in all biopsies in which at least a few germinal cells in early stages were found and in one biopsy histologically determined as Sertoli cell only. CONCLUSION(S): Our preliminary results suggest that CDY1-minor expression might increase the prospect for complete spermatogenesis, while RBM and DAZ expression can only be indicative of the presence of germinal cells. FAU - Kleiman, S E AU - Kleiman SE AD - Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, The Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. ser@tasmc.health.gov.il FAU - Lagziel, A AU - Lagziel A FAU - Yogev, L AU - Yogev L FAU - Botchan, A AU - Botchan A FAU - Paz, G AU - Paz G