• PMID: 7530000
• Gene Name: SRC
• Condition: Involved in rat spermatogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Involved in rat spermatogenesis

pp60c-src expression in rat spermatogenesis

Nishio H, Tokuda M, Itano T, Matsui H, Takeuchi Y, Hatase O.

A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src (mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis. FAU - Nishio, H AU - Nishio H AD - First Department of Physiology, Kagawa Medical School, Japan. FAU - Tokuda, M AU - Tokuda M FAU - Itano, T AU - Itano T FAU - Matsui, H AU - Matsui H FAU - Takeuchi, Y AU - Takeuchi Y